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sangon e675007 diaminobenzidine dab substrate kit mxb dab 0031 superscript ii reverse transcriptase takara 2690a goat serum jackson immunoresearch  (TaKaRa)


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    TaKaRa sangon e675007 diaminobenzidine dab substrate kit mxb dab 0031 superscript ii reverse transcriptase takara 2690a goat serum jackson immunoresearch
    Sangon E675007 Diaminobenzidine Dab Substrate Kit Mxb Dab 0031 Superscript Ii Reverse Transcriptase Takara 2690a Goat Serum Jackson Immunoresearch, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 736 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript-ii+kit/PrimeScript+II+Reverse+Transcriptase/pmc12225934__mmc1-33-54-66
    Average 96 stars, based on 736 article reviews
    sangon e675007 diaminobenzidine dab substrate kit mxb dab 0031 superscript ii reverse transcriptase takara 2690a goat serum jackson immunoresearch - by Bioz Stars, 2026-11
    96/100 stars

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    Concentration Assay:

    Article Title: Enhancing Ferroptosis in Lung Adenocarcinoma Cells via the Synergistic Action of Nonthermal Biocompatible Plasma and a Bioactive Phenolic Compound.
    Article Snippet: RNA was extracted in accordance with the manufacturer’s instructions using the Trizol reagent (Takara Bio Inc., Kusatsu, Japan). .. RNA concentration was measured using the Synergy HTX multileader (Biotek, Winooski, VT, USA). cDNA was generated using the Superscript II reverse transcriptase kit (Takara Bio, Inc, Kusatsu, Japan). .. IQ SYBR Green supermix (BD Biosciences, Seoul, Republic of Korea) was used in real-time PCR utilizing CFX96TM RealTime equipment (BioRad laboratory, Hercules, California, USA).

    Generated:

    Article Title: Enhancing Ferroptosis in Lung Adenocarcinoma Cells via the Synergistic Action of Nonthermal Biocompatible Plasma and a Bioactive Phenolic Compound.
    Article Snippet: RNA was extracted in accordance with the manufacturer’s instructions using the Trizol reagent (Takara Bio Inc., Kusatsu, Japan). .. RNA concentration was measured using the Synergy HTX multileader (Biotek, Winooski, VT, USA). cDNA was generated using the Superscript II reverse transcriptase kit (Takara Bio, Inc, Kusatsu, Japan). .. IQ SYBR Green supermix (BD Biosciences, Seoul, Republic of Korea) was used in real-time PCR utilizing CFX96TM RealTime equipment (BioRad laboratory, Hercules, California, USA).

    Reverse Transcription:

    Article Title: Enhancing Ferroptosis in Lung Adenocarcinoma Cells via the Synergistic Action of Nonthermal Biocompatible Plasma and a Bioactive Phenolic Compound.
    Article Snippet: RNA was extracted in accordance with the manufacturer’s instructions using the Trizol reagent (Takara Bio Inc., Kusatsu, Japan). .. RNA concentration was measured using the Synergy HTX multileader (Biotek, Winooski, VT, USA). cDNA was generated using the Superscript II reverse transcriptase kit (Takara Bio, Inc, Kusatsu, Japan). .. IQ SYBR Green supermix (BD Biosciences, Seoul, Republic of Korea) was used in real-time PCR utilizing CFX96TM RealTime equipment (BioRad laboratory, Hercules, California, USA).

    Article Title: The bioassay tests and mass spectrometry for evaluation of xenoestrogens in tuna fish
    Article Snippet: .. Following the manufacturer’s instructions, 1 μg of total RNA was converted to a first strand of complementary DNA (cDNA) using an oligo (dT) 18 primer with superscript II reverse transcriptase kit (Takara, Japan). ..

    Article Title: Co-Expression Network Analysis Suggests PacC Transcriptional Factor Involved in Botryosphaeria dothidea Pathogenicity in Chinese Hickory
    Article Snippet: .. RNA isolation was performed using a Qiagen RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA), followed by cDNA synthesis using a Superscript IV Reverse Transcriptase cDNA Synthesis Kit (TB Green ® Premix Ex TaqTM II, Takara Bio Inc., Kusatsu, Japan) with 2 μg of template RNA. ..

    Article Title: CDK4/6 inhibitor PD-0332991 suppresses hepatocarcinogenesis by inducing senescence of hepatic tumor-initiating cells
    Article Snippet: .. Detailed information of used chemicals, peptides, and recombinant proteins Name Company Catalogue Number Hematoxylin and Eosin Staining Kit Beyotime C0105S Apoptosis Assay Kit Beyotime C1098 Cell lysis buffer Beyotime P0013 SDS-PAGE Sample Loading Buffer Beyotime P0015 Collagenase Sigma-Aldrich V900893 DAPI Abcam ab285390 BD Pharm LyseTM Lysing Buffer BD Pharmingen 555899 Neutral balsam mounting medium Sangon E675007 Diaminobenzidine (DAB) Substrate Kit MXB DAB-0031 SuperScript II Reverse Transcriptase Takara 2690A Goat serum Jackson ImmunoResearch 005-000-001 Optimal Cutting Temperature Compound Sakura 4583 Plus Chemiluminescent Substrate Thermo Scientific 34580 Modified Oil Red O Stain Kit Solarbio G1261 Mouse Liver Dissociation Kit Miltenyi 130-105-807 BD PharmingenTM Stain Buffer (FBS) BD Pharmingen 554656 NTBC aladdin 104206-65-7 Insulin Sigma-Aldrich I0908 Dexamethasome Sigma-Aldrich D1756 mEGF MCE HY-P7067 L-glutamine Sigma-Aldrich G7513 Penicillin-Streptomycin, Liquid Gibco 15140122 DMEM Gibco C11995500BT Intracellular Staining Permeabilization Wash Buffer Biolegend 421002 CD16/CD32 Monoclonal Antibody Invitrogen 14-0161-82 Palbociclib LC Laboratories P-7766 Bovine Serum Albumin Sigma-Aldrich V900933 4% Paraformaldehyde Fix Solution Sangon Biotech E672002-0500 EDTA Antigen Retrieval Solution MXB MVS-0099 Citrate Antigen Retrieval Solution MXB MVS-0101 7-Aminoactinomycin D MCE HY-D1020 Trizol Invitrogen 15596026 Certified Fetal Bovine Serum Vivacell C04001-500 Protease Inhibitor Cocktail (EDTA- ApexBio K1007 Free,100X in DMSO) Phosphatase Inhibitor Cocktail ApexBio K1015 Takara.RR430B TB Green Fast qPCR Mix Takara Takara.RR430 B PAGE Gel Fast Preparation Kit Epizyme PG113 PageRuler Prestained Protein Ladder Thermo Scientific 26616 Primary Antibody Dilution Buffer Beyotime P0023A Secondary Antibody Dilution Buffer Beyotime P0023D Triton X-100 Sigma-Aldrich T8787 Senescence-Associated-βGalactosidase(SA-β-Gal) Stain Kit Solarbio G1580 Cellular Senescence Detection Kit - SPiDER-βGal Dojindo SG03 ..

    Article Title: Genome-wide characterization of auxin response factor ( ARF ) genes in bermudagrass and ectopically functional analysis of CdARF6-B2 gene in Arabidopsis .
    Article Snippet: Auxin response factors (ARFs) are important transcription factors that regulate the expression of auxin response genes, thus play crucial roles in plant growth and development.. However, the functions of ARF genes in bermudagrass (Cynodon dactylon L.), a turfgrass species of great economic value, remain poorly understood.. In this study, a total of 86 CdARF genes were identified from the C. dactylon genome and were categorized into five groups according to their phylogenetic relationships.

    Article Title: Genome-wide analysis of CBL and CIPK gene families in bermudagrass reveals the CdCIPK29-A1 as a stem growth angle regulator.
    Article Snippet: Key message Genome-wide analysis of CBL and CIPK gene family was conducted in bermudagrass while a functional role in stem growth angle regulation was established for CdCIPK29-A1 via the generation of molecularly modified Arabidopsis plants.. Abstract Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) are plant-specific Ca2+ sensors and effectors which mediate diverse Ca2+ signaling transduction pathways in plant growth, development, and stress responses.. However, the functions of CBLs and CIPKs in bermudagrass (Cynodon dactylon L.), a widely planted warm-season turfgrass species with great economic value, remain poorly understood.

    other:

    Article Title: Enhancing Ferroptosis in Lung Adenocarcinoma Cells via the Synergistic Action of Nonthermal Biocompatible Plasma and a Bioactive Phenolic Compound
    Article Snippet: Following cell staining, the samples were collected and subjected to instant analysis using a FACSVerse apparatus (BD Biosciences, Seoul, Republic of Korea).

    Isolation:

    Article Title: Co-Expression Network Analysis Suggests PacC Transcriptional Factor Involved in Botryosphaeria dothidea Pathogenicity in Chinese Hickory
    Article Snippet: .. RNA isolation was performed using a Qiagen RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA), followed by cDNA synthesis using a Superscript IV Reverse Transcriptase cDNA Synthesis Kit (TB Green ® Premix Ex TaqTM II, Takara Bio Inc., Kusatsu, Japan) with 2 μg of template RNA. ..

    cDNA Synthesis:

    Article Title: Co-Expression Network Analysis Suggests PacC Transcriptional Factor Involved in Botryosphaeria dothidea Pathogenicity in Chinese Hickory
    Article Snippet: .. RNA isolation was performed using a Qiagen RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA), followed by cDNA synthesis using a Superscript IV Reverse Transcriptase cDNA Synthesis Kit (TB Green ® Premix Ex TaqTM II, Takara Bio Inc., Kusatsu, Japan) with 2 μg of template RNA. ..

    Recombinant:

    Article Title: CDK4/6 inhibitor PD-0332991 suppresses hepatocarcinogenesis by inducing senescence of hepatic tumor-initiating cells
    Article Snippet: .. Detailed information of used chemicals, peptides, and recombinant proteins Name Company Catalogue Number Hematoxylin and Eosin Staining Kit Beyotime C0105S Apoptosis Assay Kit Beyotime C1098 Cell lysis buffer Beyotime P0013 SDS-PAGE Sample Loading Buffer Beyotime P0015 Collagenase Sigma-Aldrich V900893 DAPI Abcam ab285390 BD Pharm LyseTM Lysing Buffer BD Pharmingen 555899 Neutral balsam mounting medium Sangon E675007 Diaminobenzidine (DAB) Substrate Kit MXB DAB-0031 SuperScript II Reverse Transcriptase Takara 2690A Goat serum Jackson ImmunoResearch 005-000-001 Optimal Cutting Temperature Compound Sakura 4583 Plus Chemiluminescent Substrate Thermo Scientific 34580 Modified Oil Red O Stain Kit Solarbio G1261 Mouse Liver Dissociation Kit Miltenyi 130-105-807 BD PharmingenTM Stain Buffer (FBS) BD Pharmingen 554656 NTBC aladdin 104206-65-7 Insulin Sigma-Aldrich I0908 Dexamethasome Sigma-Aldrich D1756 mEGF MCE HY-P7067 L-glutamine Sigma-Aldrich G7513 Penicillin-Streptomycin, Liquid Gibco 15140122 DMEM Gibco C11995500BT Intracellular Staining Permeabilization Wash Buffer Biolegend 421002 CD16/CD32 Monoclonal Antibody Invitrogen 14-0161-82 Palbociclib LC Laboratories P-7766 Bovine Serum Albumin Sigma-Aldrich V900933 4% Paraformaldehyde Fix Solution Sangon Biotech E672002-0500 EDTA Antigen Retrieval Solution MXB MVS-0099 Citrate Antigen Retrieval Solution MXB MVS-0101 7-Aminoactinomycin D MCE HY-D1020 Trizol Invitrogen 15596026 Certified Fetal Bovine Serum Vivacell C04001-500 Protease Inhibitor Cocktail (EDTA- ApexBio K1007 Free,100X in DMSO) Phosphatase Inhibitor Cocktail ApexBio K1015 Takara.RR430B TB Green Fast qPCR Mix Takara Takara.RR430 B PAGE Gel Fast Preparation Kit Epizyme PG113 PageRuler Prestained Protein Ladder Thermo Scientific 26616 Primary Antibody Dilution Buffer Beyotime P0023A Secondary Antibody Dilution Buffer Beyotime P0023D Triton X-100 Sigma-Aldrich T8787 Senescence-Associated-βGalactosidase(SA-β-Gal) Stain Kit Solarbio G1580 Cellular Senescence Detection Kit - SPiDER-βGal Dojindo SG03 ..

    Staining:

    Article Title: CDK4/6 inhibitor PD-0332991 suppresses hepatocarcinogenesis by inducing senescence of hepatic tumor-initiating cells
    Article Snippet: .. Detailed information of used chemicals, peptides, and recombinant proteins Name Company Catalogue Number Hematoxylin and Eosin Staining Kit Beyotime C0105S Apoptosis Assay Kit Beyotime C1098 Cell lysis buffer Beyotime P0013 SDS-PAGE Sample Loading Buffer Beyotime P0015 Collagenase Sigma-Aldrich V900893 DAPI Abcam ab285390 BD Pharm LyseTM Lysing Buffer BD Pharmingen 555899 Neutral balsam mounting medium Sangon E675007 Diaminobenzidine (DAB) Substrate Kit MXB DAB-0031 SuperScript II Reverse Transcriptase Takara 2690A Goat serum Jackson ImmunoResearch 005-000-001 Optimal Cutting Temperature Compound Sakura 4583 Plus Chemiluminescent Substrate Thermo Scientific 34580 Modified Oil Red O Stain Kit Solarbio G1261 Mouse Liver Dissociation Kit Miltenyi 130-105-807 BD PharmingenTM Stain Buffer (FBS) BD Pharmingen 554656 NTBC aladdin 104206-65-7 Insulin Sigma-Aldrich I0908 Dexamethasome Sigma-Aldrich D1756 mEGF MCE HY-P7067 L-glutamine Sigma-Aldrich G7513 Penicillin-Streptomycin, Liquid Gibco 15140122 DMEM Gibco C11995500BT Intracellular Staining Permeabilization Wash Buffer Biolegend 421002 CD16/CD32 Monoclonal Antibody Invitrogen 14-0161-82 Palbociclib LC Laboratories P-7766 Bovine Serum Albumin Sigma-Aldrich V900933 4% Paraformaldehyde Fix Solution Sangon Biotech E672002-0500 EDTA Antigen Retrieval Solution MXB MVS-0099 Citrate Antigen Retrieval Solution MXB MVS-0101 7-Aminoactinomycin D MCE HY-D1020 Trizol Invitrogen 15596026 Certified Fetal Bovine Serum Vivacell C04001-500 Protease Inhibitor Cocktail (EDTA- ApexBio K1007 Free,100X in DMSO) Phosphatase Inhibitor Cocktail ApexBio K1015 Takara.RR430B TB Green Fast qPCR Mix Takara Takara.RR430 B PAGE Gel Fast Preparation Kit Epizyme PG113 PageRuler Prestained Protein Ladder Thermo Scientific 26616 Primary Antibody Dilution Buffer Beyotime P0023A Secondary Antibody Dilution Buffer Beyotime P0023D Triton X-100 Sigma-Aldrich T8787 Senescence-Associated-βGalactosidase(SA-β-Gal) Stain Kit Solarbio G1580 Cellular Senescence Detection Kit - SPiDER-βGal Dojindo SG03 ..

    Apoptosis Assay:

    Article Title: CDK4/6 inhibitor PD-0332991 suppresses hepatocarcinogenesis by inducing senescence of hepatic tumor-initiating cells
    Article Snippet: .. Detailed information of used chemicals, peptides, and recombinant proteins Name Company Catalogue Number Hematoxylin and Eosin Staining Kit Beyotime C0105S Apoptosis Assay Kit Beyotime C1098 Cell lysis buffer Beyotime P0013 SDS-PAGE Sample Loading Buffer Beyotime P0015 Collagenase Sigma-Aldrich V900893 DAPI Abcam ab285390 BD Pharm LyseTM Lysing Buffer BD Pharmingen 555899 Neutral balsam mounting medium Sangon E675007 Diaminobenzidine (DAB) Substrate Kit MXB DAB-0031 SuperScript II Reverse Transcriptase Takara 2690A Goat serum Jackson ImmunoResearch 005-000-001 Optimal Cutting Temperature Compound Sakura 4583 Plus Chemiluminescent Substrate Thermo Scientific 34580 Modified Oil Red O Stain Kit Solarbio G1261 Mouse Liver Dissociation Kit Miltenyi 130-105-807 BD PharmingenTM Stain Buffer (FBS) BD Pharmingen 554656 NTBC aladdin 104206-65-7 Insulin Sigma-Aldrich I0908 Dexamethasome Sigma-Aldrich D1756 mEGF MCE HY-P7067 L-glutamine Sigma-Aldrich G7513 Penicillin-Streptomycin, Liquid Gibco 15140122 DMEM Gibco C11995500BT Intracellular Staining Permeabilization Wash Buffer Biolegend 421002 CD16/CD32 Monoclonal Antibody Invitrogen 14-0161-82 Palbociclib LC Laboratories P-7766 Bovine Serum Albumin Sigma-Aldrich V900933 4% Paraformaldehyde Fix Solution Sangon Biotech E672002-0500 EDTA Antigen Retrieval Solution MXB MVS-0099 Citrate Antigen Retrieval Solution MXB MVS-0101 7-Aminoactinomycin D MCE HY-D1020 Trizol Invitrogen 15596026 Certified Fetal Bovine Serum Vivacell C04001-500 Protease Inhibitor Cocktail (EDTA- ApexBio K1007 Free,100X in DMSO) Phosphatase Inhibitor Cocktail ApexBio K1015 Takara.RR430B TB Green Fast qPCR Mix Takara Takara.RR430 B PAGE Gel Fast Preparation Kit Epizyme PG113 PageRuler Prestained Protein Ladder Thermo Scientific 26616 Primary Antibody Dilution Buffer Beyotime P0023A Secondary Antibody Dilution Buffer Beyotime P0023D Triton X-100 Sigma-Aldrich T8787 Senescence-Associated-βGalactosidase(SA-β-Gal) Stain Kit Solarbio G1580 Cellular Senescence Detection Kit - SPiDER-βGal Dojindo SG03 ..

    Lysis:

    Article Title: CDK4/6 inhibitor PD-0332991 suppresses hepatocarcinogenesis by inducing senescence of hepatic tumor-initiating cells
    Article Snippet: .. Detailed information of used chemicals, peptides, and recombinant proteins Name Company Catalogue Number Hematoxylin and Eosin Staining Kit Beyotime C0105S Apoptosis Assay Kit Beyotime C1098 Cell lysis buffer Beyotime P0013 SDS-PAGE Sample Loading Buffer Beyotime P0015 Collagenase Sigma-Aldrich V900893 DAPI Abcam ab285390 BD Pharm LyseTM Lysing Buffer BD Pharmingen 555899 Neutral balsam mounting medium Sangon E675007 Diaminobenzidine (DAB) Substrate Kit MXB DAB-0031 SuperScript II Reverse Transcriptase Takara 2690A Goat serum Jackson ImmunoResearch 005-000-001 Optimal Cutting Temperature Compound Sakura 4583 Plus Chemiluminescent Substrate Thermo Scientific 34580 Modified Oil Red O Stain Kit Solarbio G1261 Mouse Liver Dissociation Kit Miltenyi 130-105-807 BD PharmingenTM Stain Buffer (FBS) BD Pharmingen 554656 NTBC aladdin 104206-65-7 Insulin Sigma-Aldrich I0908 Dexamethasome Sigma-Aldrich D1756 mEGF MCE HY-P7067 L-glutamine Sigma-Aldrich G7513 Penicillin-Streptomycin, Liquid Gibco 15140122 DMEM Gibco C11995500BT Intracellular Staining Permeabilization Wash Buffer Biolegend 421002 CD16/CD32 Monoclonal Antibody Invitrogen 14-0161-82 Palbociclib LC Laboratories P-7766 Bovine Serum Albumin Sigma-Aldrich V900933 4% Paraformaldehyde Fix Solution Sangon Biotech E672002-0500 EDTA Antigen Retrieval Solution MXB MVS-0099 Citrate Antigen Retrieval Solution MXB MVS-0101 7-Aminoactinomycin D MCE HY-D1020 Trizol Invitrogen 15596026 Certified Fetal Bovine Serum Vivacell C04001-500 Protease Inhibitor Cocktail (EDTA- ApexBio K1007 Free,100X in DMSO) Phosphatase Inhibitor Cocktail ApexBio K1015 Takara.RR430B TB Green Fast qPCR Mix Takara Takara.RR430 B PAGE Gel Fast Preparation Kit Epizyme PG113 PageRuler Prestained Protein Ladder Thermo Scientific 26616 Primary Antibody Dilution Buffer Beyotime P0023A Secondary Antibody Dilution Buffer Beyotime P0023D Triton X-100 Sigma-Aldrich T8787 Senescence-Associated-βGalactosidase(SA-β-Gal) Stain Kit Solarbio G1580 Cellular Senescence Detection Kit - SPiDER-βGal Dojindo SG03 ..

    SDS Page:

    Article Title: CDK4/6 inhibitor PD-0332991 suppresses hepatocarcinogenesis by inducing senescence of hepatic tumor-initiating cells
    Article Snippet: .. Detailed information of used chemicals, peptides, and recombinant proteins Name Company Catalogue Number Hematoxylin and Eosin Staining Kit Beyotime C0105S Apoptosis Assay Kit Beyotime C1098 Cell lysis buffer Beyotime P0013 SDS-PAGE Sample Loading Buffer Beyotime P0015 Collagenase Sigma-Aldrich V900893 DAPI Abcam ab285390 BD Pharm LyseTM Lysing Buffer BD Pharmingen 555899 Neutral balsam mounting medium Sangon E675007 Diaminobenzidine (DAB) Substrate Kit MXB DAB-0031 SuperScript II Reverse Transcriptase Takara 2690A Goat serum Jackson ImmunoResearch 005-000-001 Optimal Cutting Temperature Compound Sakura 4583 Plus Chemiluminescent Substrate Thermo Scientific 34580 Modified Oil Red O Stain Kit Solarbio G1261 Mouse Liver Dissociation Kit Miltenyi 130-105-807 BD PharmingenTM Stain Buffer (FBS) BD Pharmingen 554656 NTBC aladdin 104206-65-7 Insulin Sigma-Aldrich I0908 Dexamethasome Sigma-Aldrich D1756 mEGF MCE HY-P7067 L-glutamine Sigma-Aldrich G7513 Penicillin-Streptomycin, Liquid Gibco 15140122 DMEM Gibco C11995500BT Intracellular Staining Permeabilization Wash Buffer Biolegend 421002 CD16/CD32 Monoclonal Antibody Invitrogen 14-0161-82 Palbociclib LC Laboratories P-7766 Bovine Serum Albumin Sigma-Aldrich V900933 4% Paraformaldehyde Fix Solution Sangon Biotech E672002-0500 EDTA Antigen Retrieval Solution MXB MVS-0099 Citrate Antigen Retrieval Solution MXB MVS-0101 7-Aminoactinomycin D MCE HY-D1020 Trizol Invitrogen 15596026 Certified Fetal Bovine Serum Vivacell C04001-500 Protease Inhibitor Cocktail (EDTA- ApexBio K1007 Free,100X in DMSO) Phosphatase Inhibitor Cocktail ApexBio K1015 Takara.RR430B TB Green Fast qPCR Mix Takara Takara.RR430 B PAGE Gel Fast Preparation Kit Epizyme PG113 PageRuler Prestained Protein Ladder Thermo Scientific 26616 Primary Antibody Dilution Buffer Beyotime P0023A Secondary Antibody Dilution Buffer Beyotime P0023D Triton X-100 Sigma-Aldrich T8787 Senescence-Associated-βGalactosidase(SA-β-Gal) Stain Kit Solarbio G1580 Cellular Senescence Detection Kit - SPiDER-βGal Dojindo SG03 ..

    Modification:

    Article Title: CDK4/6 inhibitor PD-0332991 suppresses hepatocarcinogenesis by inducing senescence of hepatic tumor-initiating cells
    Article Snippet: .. Detailed information of used chemicals, peptides, and recombinant proteins Name Company Catalogue Number Hematoxylin and Eosin Staining Kit Beyotime C0105S Apoptosis Assay Kit Beyotime C1098 Cell lysis buffer Beyotime P0013 SDS-PAGE Sample Loading Buffer Beyotime P0015 Collagenase Sigma-Aldrich V900893 DAPI Abcam ab285390 BD Pharm LyseTM Lysing Buffer BD Pharmingen 555899 Neutral balsam mounting medium Sangon E675007 Diaminobenzidine (DAB) Substrate Kit MXB DAB-0031 SuperScript II Reverse Transcriptase Takara 2690A Goat serum Jackson ImmunoResearch 005-000-001 Optimal Cutting Temperature Compound Sakura 4583 Plus Chemiluminescent Substrate Thermo Scientific 34580 Modified Oil Red O Stain Kit Solarbio G1261 Mouse Liver Dissociation Kit Miltenyi 130-105-807 BD PharmingenTM Stain Buffer (FBS) BD Pharmingen 554656 NTBC aladdin 104206-65-7 Insulin Sigma-Aldrich I0908 Dexamethasome Sigma-Aldrich D1756 mEGF MCE HY-P7067 L-glutamine Sigma-Aldrich G7513 Penicillin-Streptomycin, Liquid Gibco 15140122 DMEM Gibco C11995500BT Intracellular Staining Permeabilization Wash Buffer Biolegend 421002 CD16/CD32 Monoclonal Antibody Invitrogen 14-0161-82 Palbociclib LC Laboratories P-7766 Bovine Serum Albumin Sigma-Aldrich V900933 4% Paraformaldehyde Fix Solution Sangon Biotech E672002-0500 EDTA Antigen Retrieval Solution MXB MVS-0099 Citrate Antigen Retrieval Solution MXB MVS-0101 7-Aminoactinomycin D MCE HY-D1020 Trizol Invitrogen 15596026 Certified Fetal Bovine Serum Vivacell C04001-500 Protease Inhibitor Cocktail (EDTA- ApexBio K1007 Free,100X in DMSO) Phosphatase Inhibitor Cocktail ApexBio K1015 Takara.RR430B TB Green Fast qPCR Mix Takara Takara.RR430 B PAGE Gel Fast Preparation Kit Epizyme PG113 PageRuler Prestained Protein Ladder Thermo Scientific 26616 Primary Antibody Dilution Buffer Beyotime P0023A Secondary Antibody Dilution Buffer Beyotime P0023D Triton X-100 Sigma-Aldrich T8787 Senescence-Associated-βGalactosidase(SA-β-Gal) Stain Kit Solarbio G1580 Cellular Senescence Detection Kit - SPiDER-βGal Dojindo SG03 ..

    Protease Inhibitor:

    Article Title: CDK4/6 inhibitor PD-0332991 suppresses hepatocarcinogenesis by inducing senescence of hepatic tumor-initiating cells
    Article Snippet: .. Detailed information of used chemicals, peptides, and recombinant proteins Name Company Catalogue Number Hematoxylin and Eosin Staining Kit Beyotime C0105S Apoptosis Assay Kit Beyotime C1098 Cell lysis buffer Beyotime P0013 SDS-PAGE Sample Loading Buffer Beyotime P0015 Collagenase Sigma-Aldrich V900893 DAPI Abcam ab285390 BD Pharm LyseTM Lysing Buffer BD Pharmingen 555899 Neutral balsam mounting medium Sangon E675007 Diaminobenzidine (DAB) Substrate Kit MXB DAB-0031 SuperScript II Reverse Transcriptase Takara 2690A Goat serum Jackson ImmunoResearch 005-000-001 Optimal Cutting Temperature Compound Sakura 4583 Plus Chemiluminescent Substrate Thermo Scientific 34580 Modified Oil Red O Stain Kit Solarbio G1261 Mouse Liver Dissociation Kit Miltenyi 130-105-807 BD PharmingenTM Stain Buffer (FBS) BD Pharmingen 554656 NTBC aladdin 104206-65-7 Insulin Sigma-Aldrich I0908 Dexamethasome Sigma-Aldrich D1756 mEGF MCE HY-P7067 L-glutamine Sigma-Aldrich G7513 Penicillin-Streptomycin, Liquid Gibco 15140122 DMEM Gibco C11995500BT Intracellular Staining Permeabilization Wash Buffer Biolegend 421002 CD16/CD32 Monoclonal Antibody Invitrogen 14-0161-82 Palbociclib LC Laboratories P-7766 Bovine Serum Albumin Sigma-Aldrich V900933 4% Paraformaldehyde Fix Solution Sangon Biotech E672002-0500 EDTA Antigen Retrieval Solution MXB MVS-0099 Citrate Antigen Retrieval Solution MXB MVS-0101 7-Aminoactinomycin D MCE HY-D1020 Trizol Invitrogen 15596026 Certified Fetal Bovine Serum Vivacell C04001-500 Protease Inhibitor Cocktail (EDTA- ApexBio K1007 Free,100X in DMSO) Phosphatase Inhibitor Cocktail ApexBio K1015 Takara.RR430B TB Green Fast qPCR Mix Takara Takara.RR430 B PAGE Gel Fast Preparation Kit Epizyme PG113 PageRuler Prestained Protein Ladder Thermo Scientific 26616 Primary Antibody Dilution Buffer Beyotime P0023A Secondary Antibody Dilution Buffer Beyotime P0023D Triton X-100 Sigma-Aldrich T8787 Senescence-Associated-βGalactosidase(SA-β-Gal) Stain Kit Solarbio G1580 Cellular Senescence Detection Kit - SPiDER-βGal Dojindo SG03 ..

    Real-time Polymerase Chain Reaction:

    Article Title: CDK4/6 inhibitor PD-0332991 suppresses hepatocarcinogenesis by inducing senescence of hepatic tumor-initiating cells
    Article Snippet: .. Detailed information of used chemicals, peptides, and recombinant proteins Name Company Catalogue Number Hematoxylin and Eosin Staining Kit Beyotime C0105S Apoptosis Assay Kit Beyotime C1098 Cell lysis buffer Beyotime P0013 SDS-PAGE Sample Loading Buffer Beyotime P0015 Collagenase Sigma-Aldrich V900893 DAPI Abcam ab285390 BD Pharm LyseTM Lysing Buffer BD Pharmingen 555899 Neutral balsam mounting medium Sangon E675007 Diaminobenzidine (DAB) Substrate Kit MXB DAB-0031 SuperScript II Reverse Transcriptase Takara 2690A Goat serum Jackson ImmunoResearch 005-000-001 Optimal Cutting Temperature Compound Sakura 4583 Plus Chemiluminescent Substrate Thermo Scientific 34580 Modified Oil Red O Stain Kit Solarbio G1261 Mouse Liver Dissociation Kit Miltenyi 130-105-807 BD PharmingenTM Stain Buffer (FBS) BD Pharmingen 554656 NTBC aladdin 104206-65-7 Insulin Sigma-Aldrich I0908 Dexamethasome Sigma-Aldrich D1756 mEGF MCE HY-P7067 L-glutamine Sigma-Aldrich G7513 Penicillin-Streptomycin, Liquid Gibco 15140122 DMEM Gibco C11995500BT Intracellular Staining Permeabilization Wash Buffer Biolegend 421002 CD16/CD32 Monoclonal Antibody Invitrogen 14-0161-82 Palbociclib LC Laboratories P-7766 Bovine Serum Albumin Sigma-Aldrich V900933 4% Paraformaldehyde Fix Solution Sangon Biotech E672002-0500 EDTA Antigen Retrieval Solution MXB MVS-0099 Citrate Antigen Retrieval Solution MXB MVS-0101 7-Aminoactinomycin D MCE HY-D1020 Trizol Invitrogen 15596026 Certified Fetal Bovine Serum Vivacell C04001-500 Protease Inhibitor Cocktail (EDTA- ApexBio K1007 Free,100X in DMSO) Phosphatase Inhibitor Cocktail ApexBio K1015 Takara.RR430B TB Green Fast qPCR Mix Takara Takara.RR430 B PAGE Gel Fast Preparation Kit Epizyme PG113 PageRuler Prestained Protein Ladder Thermo Scientific 26616 Primary Antibody Dilution Buffer Beyotime P0023A Secondary Antibody Dilution Buffer Beyotime P0023D Triton X-100 Sigma-Aldrich T8787 Senescence-Associated-βGalactosidase(SA-β-Gal) Stain Kit Solarbio G1580 Cellular Senescence Detection Kit - SPiDER-βGal Dojindo SG03 ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: CDK4/6 inhibitor PD-0332991 suppresses hepatocarcinogenesis by inducing senescence of hepatic tumor-initiating cells
    Article Snippet: .. Detailed information of used chemicals, peptides, and recombinant proteins Name Company Catalogue Number Hematoxylin and Eosin Staining Kit Beyotime C0105S Apoptosis Assay Kit Beyotime C1098 Cell lysis buffer Beyotime P0013 SDS-PAGE Sample Loading Buffer Beyotime P0015 Collagenase Sigma-Aldrich V900893 DAPI Abcam ab285390 BD Pharm LyseTM Lysing Buffer BD Pharmingen 555899 Neutral balsam mounting medium Sangon E675007 Diaminobenzidine (DAB) Substrate Kit MXB DAB-0031 SuperScript II Reverse Transcriptase Takara 2690A Goat serum Jackson ImmunoResearch 005-000-001 Optimal Cutting Temperature Compound Sakura 4583 Plus Chemiluminescent Substrate Thermo Scientific 34580 Modified Oil Red O Stain Kit Solarbio G1261 Mouse Liver Dissociation Kit Miltenyi 130-105-807 BD PharmingenTM Stain Buffer (FBS) BD Pharmingen 554656 NTBC aladdin 104206-65-7 Insulin Sigma-Aldrich I0908 Dexamethasome Sigma-Aldrich D1756 mEGF MCE HY-P7067 L-glutamine Sigma-Aldrich G7513 Penicillin-Streptomycin, Liquid Gibco 15140122 DMEM Gibco C11995500BT Intracellular Staining Permeabilization Wash Buffer Biolegend 421002 CD16/CD32 Monoclonal Antibody Invitrogen 14-0161-82 Palbociclib LC Laboratories P-7766 Bovine Serum Albumin Sigma-Aldrich V900933 4% Paraformaldehyde Fix Solution Sangon Biotech E672002-0500 EDTA Antigen Retrieval Solution MXB MVS-0099 Citrate Antigen Retrieval Solution MXB MVS-0101 7-Aminoactinomycin D MCE HY-D1020 Trizol Invitrogen 15596026 Certified Fetal Bovine Serum Vivacell C04001-500 Protease Inhibitor Cocktail (EDTA- ApexBio K1007 Free,100X in DMSO) Phosphatase Inhibitor Cocktail ApexBio K1015 Takara.RR430B TB Green Fast qPCR Mix Takara Takara.RR430 B PAGE Gel Fast Preparation Kit Epizyme PG113 PageRuler Prestained Protein Ladder Thermo Scientific 26616 Primary Antibody Dilution Buffer Beyotime P0023A Secondary Antibody Dilution Buffer Beyotime P0023D Triton X-100 Sigma-Aldrich T8787 Senescence-Associated-βGalactosidase(SA-β-Gal) Stain Kit Solarbio G1580 Cellular Senescence Detection Kit - SPiDER-βGal Dojindo SG03 ..

    Synthesized:

    Article Title: Genome-wide characterization of auxin response factor ( ARF ) genes in bermudagrass and ectopically functional analysis of CdARF6-B2 gene in Arabidopsis .
    Article Snippet: Auxin response factors (ARFs) are important transcription factors that regulate the expression of auxin response genes, thus play crucial roles in plant growth and development.. However, the functions of ARF genes in bermudagrass (Cynodon dactylon L.), a turfgrass species of great economic value, remain poorly understood.. In this study, a total of 86 CdARF genes were identified from the C. dactylon genome and were categorized into five groups according to their phylogenetic relationships.

    Article Title: Genome-wide analysis of CBL and CIPK gene families in bermudagrass reveals the CdCIPK29-A1 as a stem growth angle regulator.
    Article Snippet: Key message Genome-wide analysis of CBL and CIPK gene family was conducted in bermudagrass while a functional role in stem growth angle regulation was established for CdCIPK29-A1 via the generation of molecularly modified Arabidopsis plants.. Abstract Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) are plant-specific Ca2+ sensors and effectors which mediate diverse Ca2+ signaling transduction pathways in plant growth, development, and stress responses.. However, the functions of CBLs and CIPKs in bermudagrass (Cynodon dactylon L.), a widely planted warm-season turfgrass species with great economic value, remain poorly understood.



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